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【Objective】 To explore the effects of different Chinese herbal medicine feed additives on growth performance, serum physiological and biochemical indexes, intestinal digestive enzyme activity, liver antioxidant immune function and intestinal flora structure of pearl gentian grouper(Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂), and to provide theoretical basis for the application of Chinese herbal medicine feed additives in the healthy breeding of pearl gentian grouper. 【Method】 A feeding trial lasting 56 days was conducted using the pearl gentian grouper with initial body mass of(17.64 ± 0.20) g as the research object, and seven treatment groups were set up, including control group, 1%(implying mass fraction in feed, the same below) Rabdosiae Rubescentis Herba(Rabdosia rubescens) group, 2% R. rubescens group, 1% R. rubescens compound group, 2% R. rubescens compound group, 1% Xiaoyao San group and 2% Xiaoyao San group, with three replicates in each group and 15 fish in each replicate. The growth performance, body shape index, serum physiological and biochemical indexes, intestinal digestive enzyme activity, liver antioxidant and non-specific immune indexes of the fish were measured, and the composition of intestinal flora was analyzed by 16S rRNA high-throughput sequencing. 【Result】 Different Chinese herbal medicine additives had significant effects on the growth performance, digestive ability, antioxidant immune status and intestinal flora structure of pearl gentian grouper. Compared with the control group, the final average mass, mass gain rate and specific growth rate of 1% R. rubescens group, 1% R. rubescens compound group, 2% R. rubescens compound group and 1% Xiaoyao San group were significantly increased. Serum biochemical indicators showed that the activities of alanine aminotransferase and aspartate aminotransferase in each Chinese herbal medicine addition group were significantly lower than those in the control group. The results of digestive enzyme showed that the amylase activity of 1% R. rubescens compound group, 2% R. rubescens compound group, 1% Xiaoyao San group, 2% Xiaoyao San group and 2% R. rubescens group was significantly higher than that of 1% R. rubescens group and control group. The pepsin activity of 2% R. rubescens compound group was significantly higher than that of 1% R. rubescens group, 2% R. rubescens group, 1% Xiaoyao San group and control group. The liver antioxidant and immune indexes showed that the catalase activity of 1% R. rubescens group, 2% R. rubescens group and 2% Xiaoyao San group were significantly increased. The activity of superoxide dismutase in 2% R. rubescens compound group and 2% Xiaoyao San group were significantly higher than that in other treatment groups. At the same time, the content of malondialdehyde in the 2% R. rubescens compound group was significantly lower than that in the control group. The analysis of intestinal flora showed that the richness of intestinal flora in the Chinese herbal medicine addition groups were higher than that in the control group, and there were differences in the structure of intestinal flora in each group. At the genus level, the abundance of Lactococcus in Xiaoyao San group was higher, the abundance of Bacillus in R. rubescens group was higher, and the abundance of Vibrio in 1% R. rubescens group, 1% R. rubescens compound group, 2% R. rubescens compound group, 1% Xiaoyao San group and 2% Xiaoyao San group were significantly lower than that in control group. α = 0.05. 【Conclusion】 Under the conditions of this experiment, the comprehensive effect of adding 2 % R. rubescens compound Chinese herbal medicine is the best, which can be used as a potential green feed additive to promote the healthy breeding of pearl gentian grouper.
[1]陈超,孔祥迪,李炎璐,等.棕点石斑鱼(♀)×鞍带石斑鱼(♂)杂交子代胚胎及仔稚幼鱼发育的跟踪观察[J].渔业科学进展, 2014, 35(5):135-144.
[22]APINES-AMAR M J S, AMAR E C, FAISAN J P, et al.Dietary onion and ginger enhance growth, hematoimmunological responses, and disease resistance in brownmarbled grouper, Epinephelus fuscoguttatus[J]. Aquac Aquar Conserv Legis, 2012, 5(4), 231-239.
[3]孟晓雪,卫育良,梁萌青,等.鱼类胆固醇营养需求研究进展[J].动物营养学报, 2021, 33(2):719-728.
[4]FAHEEM M, KHALIQ S, MUSTAFA N, et al. Dietary Moringa oleferia leaf meal induce growth, innate immunity and cytokine expression in grass carp, Ctenopharyngodon idella[J]. Aquaculture Nutrition, 2020, 26(4):1164-1172.
[5]HOSEINIFAR S H, SUN Y Z, ZHOU Z, et al. Boosting immune function and disease bio-control through environment-friendly and sustainable approaches in finfish aquaculture:herbal therapy scenarios[J]. Reviews in Fisheries Science&Aquaculture, 2020, 28(3):303-321.
[6]ABARIKE E D, JIAN J C, TANG J F, et al. Traditional Chinese medicine enhances growth, immune response,and resistance to Streptococcus agalactiae in Nile tilapia[J]. Journal of Aquatic Animal Health, 2019, 31(1):46-55.
[7]HUANG Z F, LU J, YE Y L, et al. Effects of dietary Chinese herbal medicines mixture on growth performance,digestive enzyme activity and serum biochemical parameters of European eel, Anguilla anguilla[J]. Aquaculture Reports, 2020, 18:100510.
[8]刘建群,高俊博,刘小红.冬凌草的化学成分研究[J].中国药房, 2015, 26(33):4724-4726.
[9]郭萍,李玉山,郭远强.冬凌草化学成分和药理活性研究进展[J].药物评价研究, 2010, 33(2):144-147.
[10]肖博,周胜杰,汪迎港,等.发酵黄芪对棕点石斑鱼的生长、消化、免疫功能和抗氨氮能力的影响[J].南方水产科学, 2023, 19(2):161-169.
[11]李萍.杜仲叶提取物对鲤鱼生长性能、抗氧化性能及免疫功能的影响[J].中国饲料, 2026(2):71-74.
[12]武容,蔡菲菲,陈晓乐,等.逍遥散治疗肝郁脾虚证的应用及其作用机制的研究进展[J].中医学, 2018(3):193-200.
[13]徐创文,蒋魁,虞为,等.丁酸梭菌对花鲈幼鱼生长性能、免疫消化及肠道菌群的影响[J].南方农业学报, 2024, 55(2):366-377
[14]CHOI W M, MO W Y, WU S C, et al. Effects of traditional Chinese medicines(TCM)on the immune response of grass carp(Ctenopharyngodon idellus)[J]. Aquaculture International, 2014, 22(2):361-377.
[15]CAI J, YANG Z G, HUANG Y, et al. Effects of Chinese herbal medicines on growth performance, intestinal flora,immunity and serum metabolites of hybrid grouper(Epinephelus fuscoguttatus♀×Epinephelus lanceolatu♂)[J].Fish&Shellfish Immunology, 2023, 140:108946.
[16]黄玉章.黄芪多糖对奥尼罗非鱼生长性能和免疫功能的影响[D].福州:福建农林大学, 2009.
[17]冷向军,孟晓林,李家乐,等.杜仲叶对草鱼生长、血清非特异性免疫指标和肉质影响的初步研究[J].水产学报, 2008, 32(3):434-440.
[18]赵春彦,郭娟,李秀娟,等.齐口裂腹鱼肠道主要消化酶活性探研[J].通化师范学院学报, 2010, 31(2):42-44.
[19]PERES H, SANTOS S, OLIVA-TELES A. Lack of compensatory growth response in gilthead seabream(Sparus aurata)juveniles following starvation and subsequent refeeding[J]. Aquaculture, 2011, 318(3/4):384-388.
[20]陈文静,丁立云,邓勇辉,等.饥饿胁迫对彭泽鲫幼鱼形体指标、肌肉脂肪酸组成和肝脏脂蛋白脂酶基因表达的影响[J].动物营养学报, 2020, 32(6):2782-2790.
[21]谭晓晨,麻艳群,董升辉,等.中草药复方制剂对团头鲂生长性能及肝脏组织的影响[J].饲料研究, 2021, 44(17):39-43.
[22]吕小文,郝倩,陈业渊,等.饲料添加芒果叶黄酮浸膏促进鱼类生长[J].农业工程学报, 2013, 29(18):277-283.
[23]谭连杰.三种植物多糖对卵形鲳鲹生长性能及生化指标的影响[D].上海:上海海洋大学, 2018.
[24]程玉冰,潘庭双,侯冠军,等.不同中草药添加剂对草鱼生长及肝胆综合症的影响[J].安徽农学通报, 2008, 14(23):146.
[25]SHUBHA J R, BHATT P. Functional attributes of polyphenol-rich Woodfordia fruticosa extract:an active ingredient in traditional Indian medicine with nutraceutical potential[J]. Journal of Herbal Medicine, 2021, 29:100488.
[26]苏艳莉,戈贤平,孙盛明,等.高温季节饥饿后补偿摄食对团头鲂幼鱼生长性能、血清生化指标、肠道消化酶活性及肝脏抗氧化酶活性的影响[J].动物营养学报,2017, 29(11):4198-4206.
[27]LEWIS G F, RADER D J. New insights into the regulation of HDL metabolism and reverse cholesterol transport[J]. Circulation Research, 2005, 96(12):1221-1232.
[28]李清宏,武晋孝,韩俊文,等.复方中草药添加剂对仔猪饲料代谢动力学的研究[J].中国农学通报, 2007, 23(3):19-23.
[29]LIN S M, SHI C M, MU M M, et al. Effect of high dietary starch levels on growth, hepatic glucose metabolism, oxidative status and immune response of juvenile largemouth bass, Micropterus salmoides[J]. Fish&Shellfish Immunology, 2018, 78:121-126.
[30]CAI Y Z, LUO Q, SUN M, et al. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer[J]. Life Sciences, 2004,74(17):2157-2184.
[31]VAN DER OOST R, BEYER J, VERMEULEN N P E.Fish bioaccumulation and biomarkers in environmental risk assessment:a review[J]. Environmental Toxicology and Pharmacology, 2003, 13(2):57-149.
[32]CHEN X L, XIANG L, JIA G, et al. Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial-related genes in longissimus dorsi muscle and liver of piglets[J]. Animal Science Journal,2019, 90(8):990-998.
[33]刘志鸿,牟海津,王清印.软体动物免疫相关酶研究进展[J].海洋水产研究, 2003, 24(3):86-90.
[34]张辉,张海莲.碱性磷酸酶在水产动物中的作用[J].河北渔业, 2003(5):12-13.
[35]杨政高.复方中草药对珍珠龙胆石斑鱼生长、肠道菌群、免疫功能和血清代谢组学的影响[D].湛江:广东海洋大学, 2022.
[36]谢海生,王华雨,王连睿,等.冬凌草的研究进展及质量标志物的预测分析[J].中药新药与临床药理, 2025, 36(4):636-643.
[37]刘芳,刘培毅,郝兰芳,等.不同分离提纯工艺对冬凌草多糖提取率与免疫活性的影响[J].中药材, 2011, 34(3):449-452.
[38]SUMON T A, HUSSAIN M A, SUMON M A A, et al.Functionality and prophylactic role of probiotics in shellfish aquaculture[J]. Aquaculture Reports, 2022, 25:101220.
[39]何娇娇,王萍,冯建,等.发酵豆粕对大黄鱼生长、肠道结构及肠道微生物菌群的研究[J].水生生物学报, 2018,42(5):919-928.
[40]徐世微, Vivian Hlordzi,邹岷,等.复合蛋白源替代鱼粉对珍珠龙胆石斑鱼幼鱼生长性能、体成分和肠道菌群的影响[J].中国饲料, 2023(1):89-97.
[41]ROESELERS G, MITTGE E K, et al. Evidence for a core gut microbiota in the zebrafish[J]. The ISME Journal,2011, 5(10):1595-1608.
[42]吴杨,杨铿,黄小林,等.饲料中添加丁酸梭菌对卵形鲳鲹幼鱼生长性能和肠道菌群的影响[J].南方水产科学,2022, 18(3):155-162.
[43]BEHERA B K, BERA A K, PARIA P, et al. Identification and pathogenicity of Plesiomonas shigelloides in silver carp[J]. Aquaculture, 2018, 493:314-318.
[44]卢国柱.蜡样芽孢杆菌中有效抗菌物质的分离、纯化及初步鉴定[D].烟台:烟台大学, 2021.
[45]KAKTCHAM P M, TEMGOUA J B, NGOUFACK ZAMBOU F, et al. Quantitative analyses of the bacterial microbiota of rearing environment, tilapia and common carp cultured in earthen ponds and inhibitory activity of its lactic acid bacteria on fish spoilage and pathogenic bacteria[J]. World Journal of Microbiology&Biotechnology,2017, 33(2):32.
[46]刘娜娜.植物乳杆菌ZJ316及其发酵乳对DSS诱导小鼠结肠炎影响的研究[D].杭州:浙江工商大学, 2019.
[47]高权新,黄浩,赵秀锌,等.水产动物肠道菌群及其对宿主生长性能影响研究进展[J].生物资源, 2024, 46(6):529-537.
Basic Information:
China Classification Code:S965.334
Citation Information:
[1]WANG Feng'ang,ZHANG Dan,LI Yaqing ,et al.Effects of Different Chinese Herbal Medicines on Growth Performance, Antioxidant, Digestive Indexes and Intestinal Flora of Pearl Gentian Grouper[J].Journal of Guangdong Ocean University,2026,46(03):23-31.
Fund Information:
国家重点研发计划（2022YFD2401204）; 天津市科技计划项目（24YDTPJC00930）; 天津市教委科研计划项目（2022ZD004）; 天开高教科创园企业研发专项（23YFZXYC00017）
2026-05-15
2026-05-15